A year-long study used high-throughput sequencing (HTS) to analyze fungal exposure in urban (Leicester) and rural (Chilton) environments, identifying 272 fungal genera—nearly four times more than traditional methods. While fungal diversity was similar across locations, temperature and rainfall played a major role in shaping fungal composition. With climate change altering weather patterns, airborne fungi exposure could shift in unpredictable ways, increasing risks for asthma, allergies, and respiratory diseases.