Puffball mushroom (Bovistella utriformis) polysaccharides helped skin cells grow and repair tissue without damaging healthy cells. Human skin cells kept over 68% viability across doses from 19.5 to 10,000 µg/mL, with cell growth rising to 130% and 126% viability at 5,000 and 10,000 µg/mL, showing strong regenerative activity. In wound-closure tests, the extract achieved 40% scratch closure within 32 hours at 500 µg/mL, supporting its potential for skin-healing applications.