Shiitake mushrooms (Lentinula edodes) were used to create eco-friendly silver nanoparticles that lowered blood sugar and restored liver health in diabetic zebrafish. These mushroom-based nanoparticles also blocked key enzymes like α-amylase, showing antioxidant and anti-diabetic effects that rival standard treatments. The results suggest a promising path for next-generation, mushroom-derived diabetes therapies.